Meropenem for Carbapenemase screening

rosco_neo_tab_bact A significant reduction in the sensitivity of isolates to Meropenem is used as a screening step for the detection of carbapenemases

Carbapenemase screen using Meropenem, are you getting the right results?

In 2009 a UK laboratory (Maidstone) looked at Meropenem sensitivities of Pseudomonas aeruginosa comparing conventional paper discs against Rosco Neo-Sensitabs. The paper discs were shown to greatly overestimate resistance whilst the Rosco tabs were shown to be significantly more accurate; 

MIC
result (e-test)
Oxoid
S-I-R
Mast
S-I-R
Rosco
S-I-R
Sensitive - 23
11-11-1
12-8-3
20-3-0
Intermediate - 18
0-5-13
2-4-12
3-7-8
Resistant - 2
0-0-2
0-0-2
0-0-2

(based on BSAC Susceptibility Testing Methods V.8)

Rosco Neo-Sensitabs

Stability

Accuracy

Convenience

The variability of the Meropenem paper disc results is due to the instability of Meropenem when presented in this format. Recently the shelf-life of 'in use' cartridges has been reduced to just a few days to try and remove the risk of incorrect resistance reporting with paper discs.

Rosco Neo-Sensitabs discs containing Meropenem overcome this instability issue with their unique manufacturing and presentation format. They are so stable they are stored at room temperature for the duration of their declared shelf life with no discernible reduction in performance.

Rosco Neo-Sensitabs - Meropenem 10µg discs

No more discarding part used cartridges due to short shelf life

Rosco Meropenem discs, stock and 'in use' cartridges are stored at room temperature, shelf life in excess of 12 months

Most Neo-Sensitabs are stable at room temperature for up to 4 years. Those discs that do require refrigeration are stable at room temperature for 2 months obviating the need to refrigerate working stock on a daily basis - minimising loss of activity and therefore false resistance results.

643-12, Meropenem 10μg product page