ROSCO has developed a series of confirmatory (and screening) kits for the routine detection of ESBL producing isolates among the species Enterobacteriaceae. The choice of test kit depends on the isolate to be tested, and whether or not it produces chromosomal AmpC.
Therefore, the isolate should be identified beforehand, and assigned in to one of the two following groups:
Group 1:
E. coli, Klebsiella spp., P. mirabilis, Salmonella spp. and Shigella spp.
- Recommendation CLSI/EUCAST: Use of Cefotaxime and Ceftazidime and their combination with Clavulanate.
- If the isolate is, Cefoxitin resistant (zone < 19mm) also test for Cefepime and Cefepime+ Clavulanate as well.
Group 2:
Enterobacter spp., Serratia spp., Providencia spp., C. freundii, Morg. morganii and Hafnia alvei (all producing chromosomal AmpC)
- Recommendation EUCAST: Use Cefepime and Cefepime + Clavulanate
98014: Total ESBL Confirm Kit includes detection of all Enterobacteriaceae, including chromosomal AmpC producers.
98011: ESBL Confirm (acc. to CLSI/EUCAST) Kit includes detection of isolates in group 1.
98012: ESBL Confirm (Chrom. AmpC) Kit includes detection of isolates belonging to group 2.
Furthermore, the ESBL Screen Kit (98008) allows for the routine screening of ESBL producing bacteria in all Enterobacteriaceae.
98012: ESBL Confirm (Chrom. AmpC) Kit
It comprises two cartridges (100 tests) each of the following:
Cefepime 30 μg
Cefepime 30 μg + Clavulanate
Testing of Enterobacteriaceae Group 2 (possessing chromosomal AmpC) according to EUCAST.