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Neisseria PET

Intended Use
Neisseria PET uses chromogenic substrates to detect the presence of preformed enzymes in fresh cultures of Neisseria. The test can be used to confirm Neisseria meningitidis and Neisseria lactamica and presumptively identify Neisseria gonorrhoeae.

Background
Neisseria PET contains three non-interfering substrates: gamma-glutamyl-nitroanalide, bromo-chloro-indol-B-D- galactopyranoside and proline napthylamide. These substrates are bound to chromogens, coloured end products are released when substrates are hydrolysed.

Neisseria lactamica produces beta galactosidase, which hydrolyses bromo-chloro-indol-B-D-galactopyranoside to produce a blue coloured end product.

Neisseria meningitidis produces gamma-glutamylaminopeptidase, which hydrolyses gamma-glutamyl nitroanalide to produce a yellow coloured end product. A second reaction gives a blue/purple colour on the addition of the PEP reagent.

Neisseria gonorrhoeae produces hydroxyprolineaminopeptidase which hydrolyses proline napthylamide, the free napthylamide produces a red colour on addition of the PEP reagent.

Instructions For Use (En)

Material safety Data Sheet (En)

PHE, Standards for Microbiology Investigations: SMI ID 6: identification of Neisseria species

CDC: Identification of N. gonorrhoeae and Related Species

Specifications

  • Quantity: 25 tests
  • Product Code: CK3025

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