How to create your full workflow plasma control material based on this product:
We recommend the use of our human DNA-free plasma products to produce complete workflow controls with this product. Blend the cfDNA as necessary with the DNA depleted plasma to receive plasma materials with the necessary DNA concentrations.
These products are ideal for digital PCR and/or Next Generation Sequencing (NGS). In particular:
– Validation and development of sequencing protocols (e.g. Whole Genome Sequencing (WGS), Amplicon Sequencing) and PCR protocols
– Determination of the detectiont limit of the method
Unit Size:
400 ng (each vial)
Concentration:
20 ng/µl
Mutations:
- AKT1 – p.E17K (COSV62571334*, Substitution, c.49G>A)
- BRAF – p.V600E (COSV56056643*, Substitution, c.1799T>A)
- ERBB2 – p.Y772_A775dup (COSV54062409*, Insertion, c.2313_2324dup)
- KRAS – p.G12D (COSV55497369*, Substitution, c.35G>A)
- KRAS – p.Q61K (COSV55502066*, Substitution, c.181C>A)
- KRAS – p.A146T (COSV55501778*, Substitution, c.436G>A)
- PIK3CA – p.E545K (COSV55873239*, Substitution, c.1633G>A)
- PIK3CA – p.H1047R (COSV55873195*, Substitution, c.3140A>G)
Allele frequency:
0%; 0.1%; 1%; 5%
Buffer:
Tris-EDTA (10 mM Tris, 1 mM EDTA), pH 8.0
Storage:
2-8 °C
Expiration:
stable for 24 months from date of manufacture (as supplied)
Quality control
Fragmentation size:
Elektrophoresis-Bioanalyzer-High Sensitivity DNA Kit Agilent
Allele Frequency / copies (metrologically traceable):
dPCR
Quantification (metrologically traceable):
- UV-Vis Spectrophotometry (NIST-Reference method) measurement of total DNA
- fluorometric dsDNA measurement
Technical background
Derived from:
cell line GM24385 (HG002- NA24385 – huAA53E0)
*GRCh 38 Cosmic v90
