Recommended use:
These products are ideal for digital PCR and/or Next Generation Sequencing (NGS). In particular:
1. Validation and development of sequencing protocols (e.g. Whole Genome Sequencing (WGS), Amplicon Sequencing) and PCR protocols
2. Spike-in experiments with synthetic cfDNA
3. Control in workflow validations
4. Analyse the performance of your NGS pipeline by comparing to freely available datasets
5. Negative Control for EGFR mutations (p.G719S, p.E746_A750delELREA, p.S752_I759delSPKANKEI, p.S768I, p.V769_D770insASV, p.T790M, p.L858R, p.L861Q)
6. Negative Control for 5 Gene Multiplex mutations (AKT1 – p.E17K;BRAF – p.V600E; ERBB2 – p.Y772_A775dup; KRAS – p.G12D, p.Q61K, p.A146T; PIK3CA – p.E545K, p.H1047R)
Unit Size:
400 ng
Concentration:
20 ng/µl
Buffer:
Tris-EDTA (10 mM Tris, 1 mM EDTA), pH 8.0
Storage:
2-8 °C
Expiration:
stable for 24 months from date of manufacture (as supplied)
Quality control
Fragmentation size:
High Sensitivity DNA Kit Agilent
Allelic Frequency:
Next Generation Sequencing
Quantification:
UV-Vis Spectrophotometry (NIST-Reference method)
Technical background
Derived from:
The Genome In A Bottle (GIAB) cell line GM24385 (HG002- NA24385 – huAA53E0 – Ashkenazim son))
