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KPC, MBL & OXA48 confirm ID kit

The capability to detect ESBLs, AmpC and carbapenemases (KPC and metallo-ß-lactamases) in the laboratory is important to improve the clinical management of infections.

Disk (tablet) approximation tests detecting synergy (or antagonism) are useful to screen and detect the mentioned beta-lactamases.

  • Simple to test
  • Easy to interpret
  • Long shelflife
  • After opening, room temperature storage (≤ 25°C)
  • High sensitivity and specificity

Carbapenemase should be suspected if:

Ertapenem zone is ≤ 22mm (Enterobacteriaceae) or
Meropenem zone is ≤ 25mm (Enterobacteriaceae / P. aeruginosa).

MBL suggested if:

  • Resistance to Ceftazidime
  • No synergy between Cephlasporin & Clavulanate
  • Reduced susceptibility to Carbapenems

KPC will show:

  • Negative metallo-β-lactamase test
  • Synergy between Boronic acid and Carbapenems
  • No synergy between Cloxacillin and Carbapenems

OXA-48 like suspected if:

  • Negative results on all synergy tests
  • No zone of inhibition with Temocillin 30μg disc

Combined disc test
Apply one tablet of:
Meropenem (MRP10)
Meropenem + Phenylboronic acid (MRPBO) - KPC & AmpC inhibitor
Meropenem + Cloxacillin (MRPCX) - AmpC inhibitor
Meropenem + Dipicolinic acid (MRPDP) - metallo-β-lactamase inhibitor
Temocillin (TEMOC)
On an inoculated Mueller-Hinton agar plate (McFarland 0.5).

Interpretation

Specifications

  • Quantity: 50 tests
  • Product Code: 98015

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    Background

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