Intended Use
A rapid method is based on the identification of the hydrolysis of the beta-lactam ring of a carbapenem in the presence of an indicator.
Utilizing this principle ROSCO has developed 1 new Diatabs; Imipenem (x2)+ Bromothymol Blue. The test is performed quickly and the reading of the results is ready within 15 minutes to one hour, from the time the reaction is started. No lysis buffer needed.
Thus, applying this kit, in the routine screening for Carbapenemases, saves time and effort in the laboratory. This test kit can be used to determine the presence of an Carbapenemase producing organism in culture, positive blood culture and urine specimen.
Interpretation
A change of colour from blue to yellow indicates a positive reaction, indicating that the test strain possesses a carbapenemase. If the reaction is positive after 15 minutes or 30 min, the test is finished (it is not necessary to incubate further). The tube must be incubated for no more than 1 hour, because positive reactions may fade out.
If the test suspension is green yellow and the negative Control is blue, indicates a positive reaction. It is seen with oxacillinases from Acinetobacter.
If the test suspension is yellow and the Negative Control is green, indicates a positive result.
If the Negative Control Blue CARB shows a light-yellow colour, report the result as uninterpretable, no matter the result of Imipenem + Bromothymol Blue.