These Rapid Fermentation Tablets are developed for use with fermentative Enterobacteriaceae and are not recommended for other organisms.
ACTIVE INGREDIENTS:
Each tablet contains the respective carbohydrate in a final concentration of 5 mg. per tablet and other inert chemicals: Dipotassium phosphate, Phenol Red, Hyamine, and Sodium Chloride.
PROCEDURE:
For the correct carbohydrate selection, consult the Manual of Clinical Microbiology. Follow instructions for single or multiple tests as shown below.
SINGLE TEST METHOD
Add 0.25ml of sterile distilled water to the tube, inoculate with a loopful of the test organism and emulsify. Add oil overlay if desired, incubate at 35-37°C.
MULTIPLE TEST METHOD
Make a heavy suspension of the test organism in 1ml of sterile distilled water and add 1 drop of this suspension to each tube. Add oil overlay if desired, incubate at 35- 37°C.
INTERPRETATION OF RESULTS
The tube should be observed for colour change from red to yellow, indicating acid production. Fermentation may be apparent in 30 minutes and usually is complete within 6 hours. It is best to hold tubes for 24 hours before discarding negative tests. Gas production is shown by the appearance of bubbles near the bottom of the tube.
QUALITY CONTROL:
Rapid Fermentation Tablets should be tested with organisms which produce known reactions. Suitable positive ad negative control strains are listed in the IFU